Monthly Archives: June 2011

An alternative pathway for alphavirus entry

The study of alphavirus entry has been complicated by an inability to clearly identify a receptor and by experiments which only tangentially and indirectly examine the process, producing results that are difficult to interpret. The mechanism of entry has been widely accepted to be by endocytosis followed by acidification of the endosome resulting in virus […]

Characterization of herpes simplex virus. Part 2

Our analysis of these studies provides evidence that glycoprotein G variation is likely significant in clinical typing discrepancies and also in isolate variations. Analysis of the amino acid sequences of Y3369 and other isolates indicates that there is a shared significant variation among HSV-1 strains that alters viral antigen assay specificity. PCR analysis is likely […]

Characterization of herpes simplex virus

Determination of HSV type was done by PCR specific for the HSV pol gene using a common forward primer and type-specific reverse primers as performed by Abraham, et. al and Kimura, et al. DNA was extracted (Invitrogen PureLink viral DNA/RNA mini kit) from purified virus of HSV-1 (McIntyre strain), HSV-2 (Strain 333), and from the […]

Characterization of herpes simplex virus

Background Herpes simplex viruses exist as two major serotypes, type 1 (HSV-1) and type 2 (HSV-2). Determination of type, either HSV-1 or HSV-2, is important in accurate diagnosis and clinical control of transmission. Several tests are available for typing HSV, including a monoclonal antibody specific for glycoprotein G and several PCR assays. Findings A clinical […]

Human-specific Alu detection by PCR. Part 3

EBV is a herpesvirus associated with approximately 1% of tumours worldwide. EBV is the epitome of B lymphotropic viruses, but the spectrum of tumours associated with EBV extends to various types of human malignancies and carcinomas. Ubiquitous EBV infection in humans implies that most individuals carry EBV-infected cells. Therefore, mere detection of the virus in […]

Human-specific Alu detection by PCR. Part 2

Immunohistochemical staining showed that all induced tumors were LCA (leukocyte common antigen) positive, B-cell marker (CD20, CD79a) positive, and T-cell markers (both CD3 and CD45RO) negative. By these morphological and immunohistochemical features, those tumors can be diagnosed as human B-cell lymphomas. Furthermore, Alu-PCR showed that all of tumor tissues contained 221 bp Alu sequence. It […]

Human-specific Alu detection by PCR

DNA was extracted from tumor tissues as described in protocol. Alu PCR was conducted under the following conditions: 95°C predenaturation for 5 min, and then 30 cycles of denaturation at 94°C for 1 min, annealing at 57°C for 1 min and extension at 72°C for 1 min. Primers (sense: 5′-CAC CTG TAA TCC CAG CAG […]