Category Archives: Infections

The development of a rapid SYBR Green I-based quantitative PCR for detection of Duck circovirus

This report describes a one-step real-time polymerase chain reaction assay based on SYBR Green I for detection of a broad range of duck circovirus (DuCV). Align with all DuCV complete genome sequences and other Genus Circovirus download from the GenBank (such as goose circovirus, pigeon circovirus), the primers targets to the replicate gene of DuCV […]

Characterization of herpes simplex virus. Part 2

Our analysis of these studies provides evidence that glycoprotein G variation is likely significant in clinical typing discrepancies and also in isolate variations. Analysis of the amino acid sequences of Y3369 and other isolates indicates that there is a shared significant variation among HSV-1 strains that alters viral antigen assay specificity. PCR analysis is likely […]

Characterization of herpes simplex virus

Determination of HSV type was done by PCR specific for the HSV pol gene using a common forward primer and type-specific reverse primers as performed by Abraham, et. al and Kimura, et al. DNA was extracted (Invitrogen PureLink viral DNA/RNA mini kit) from purified virus of HSV-1 (McIntyre strain), HSV-2 (Strain 333), and from the […]

Characterization of herpes simplex virus

Background Herpes simplex viruses exist as two major serotypes, type 1 (HSV-1) and type 2 (HSV-2). Determination of type, either HSV-1 or HSV-2, is important in accurate diagnosis and clinical control of transmission. Several tests are available for typing HSV, including a monoclonal antibody specific for glycoprotein G and several PCR assays. Findings A clinical […]

Antiviral drugs and antiviral activity assays. Part 2

3.1 Complete nucleotide sequence of Korean ECV5 The Korean ECV 5 genome was sequenced (GenBank accession number HM775882) and its amino acid sequence was deduced. The genome is 7,430 nt in length, excluding the poly(A) tail. The 5′NCR contains 738 nt, followed by an ORF that encodes a viral polyprotein consisting of 2,196 codons, between […]

Antiviral drugs and antiviral activity assays

Assays of antiviral activity and cytotoxicity were evaluated by the modified sulforhodamine B (SRB) assay previously described and recently reported by Choi et al. Ribavirin was purchased from DUCHEFA (Netherlands); azidothymidine, acyclovir, amantadine, and lamivudine were purchased from Sigma-Aldrich (St. Louis, MO, USA), as was SRB. One day before infection, Vero cells were seeded onto […]

Genetic diversity of a Korean echovirus 5 isolate. Part 2

At present, some 40 antiviral drugs have been formally licensed for use in humans, mostly for treatment of human immunodeficiency virus, hepatitis B virus, and herpesvirus infections. The number of antiviral drugs that have been licensed for use in treating highly-pathogenic RNA virus infections is very limited; the current list of approved drugs includes anti-influenza […]

Genetic diversity of a Korean echovirus 5 isolate

Abstract An outbreak of echovirus 5 (ECV 5) occurred in Korea in 2006, marking the first time this virus had been identified in the country since enterovirus surveillance began in 1993. Using a sample isolated from a young male patient with aseptic meningitis, we performed sequencing of the Korean ECV 5 strain and compared it […]

Viral Phenotype and Immune Response in Primary Human Immunodeficiency Virus Type 1 Infection. Part 5

The emergence of SI T cell-line tropic variants in longterm infected individuals progressing to AIDS is well established. This overt replication of SI clones is thought to be due to an increasing qualitative immune deficiency with ongoing infection. Remarkably, in the three individuals with SI isolates described here, these isolates could be detected from the […]

Viral Phenotype and Immune Response in Primary Human Immunodeficiency Virus Type 1 Infection. Part 4

The number of clinical symptoms correlated significantly with both the duration and peak values ofCD8+ lymphocytosis (P < .05 for both comparisons in the Spearman nonparametric rank correlation test). CD8+ T cell numbers were moderately elevated in 5 of II cohort participants and within a normal range in the 6 others (data not shown). Although […]